Light and electron microscopic study of the distribution of substance P‐immunoreactive fibers and neurokinin‐1 receptors in the skin of the rat lower lip
Identifieur interne : 003209 ( Main/Exploration ); précédent : 003208; suivant : 003210Light and electron microscopic study of the distribution of substance P‐immunoreactive fibers and neurokinin‐1 receptors in the skin of the rat lower lip
Auteurs : Isabella Ruocco [Canada] ; A. Claudio Cuello [Canada] ; Ryuichi Shigemoto [Japon] ; Alfredo Ribeiro-Da-Silva [Canada]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2001-04-16.
English descriptors
- KwdEn :
Abstract
Cutaneous antidromic vasodilatation and plasma extravasation, two phenomena that occur in neurogenic inflammation, are partially blocked by substance P (SP) receptor antagonists and are known to be mediated in part by mast cell‐released substances, such as histamine, serotonin, and nitric oxide. In an attempt to provide a morphological substrate for the above phenomena, we applied light and electron microscopic immunocytochemistry to investigate the pattern of SP innervation of blood vessels and its relationship to mast cells in the skin of the rat lower lip. Furthermore, we examined the distribution of SP (neurokinin‐1) receptors and their relationship to SP‐immunoreactive (IR) fibers. Our results confirmed that SP‐IR fibers are found in cutaneous nerves and that terminal branches are observed around blood vessels and penetrating the epidermis. SP‐IR fibers also innervated hair follicles and sebaceous glands. At the ultrastructural level, SP‐IR varicosities were observed adjacent to arterioles, capillaries, venules, and mast cells. The varicosities possessed both dense core vesicles and agranular synaptic vesicles. We quantified the distance between SP‐IR varicosities and blood vessel endothelial cells. SP‐IR terminals were located within 0.23–5.99 μm from the endothelial cell layer in 82.7% of arterioles, in 90.2% of capillaries, and in 86.9% of venules. Although there was a trend for SP‐IR fibers to be located closer to the endothelium of venules, this difference was not significant. Neurokinin‐1 receptor (NK‐1r) immunoreactivity was most abundant in the upper dermis and was associated with the wall of blood vessels. NK‐1r were located in equal amounts on the walls of arterioles, capillaries, and venules that were innervated by SP‐IR fibers. The present results favor the concept of a participation of SP in cutaneous neurogenic vasodilatation and plasma extravasation both by an action on blood vessels after binding to the NK‐1r and by causing the release of substances from mast cells after diffusion through the connective tissue. J. Comp. Neurol. 432:466–480, 2001. © 2001 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/cne.1114
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002473
- to stream Istex, to step Curation: 002473
- to stream Istex, to step Checkpoint: 001038
- to stream Main, to step Merge: 003691
- to stream Main, to step Curation: 003209
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Light and electron microscopic study of the distribution of substance P‐immunoreactive fibers and neurokinin‐1 receptors in the skin of the rat lower lip</title>
<author><name sortKey="Ruocco, Isabella" sort="Ruocco, Isabella" uniqKey="Ruocco I" first="Isabella" last="Ruocco">Isabella Ruocco</name>
</author>
<author><name sortKey="Cuello, A Claudio" sort="Cuello, A Claudio" uniqKey="Cuello A" first="A. Claudio" last="Cuello">A. Claudio Cuello</name>
</author>
<author><name sortKey="Shigemoto, Ryuichi" sort="Shigemoto, Ryuichi" uniqKey="Shigemoto R" first="Ryuichi" last="Shigemoto">Ryuichi Shigemoto</name>
</author>
<author><name sortKey="Ribeiro A Ilva, Alfredo" sort="Ribeiro A Ilva, Alfredo" uniqKey="Ribeiro A Ilva A" first="Alfredo" last="Ribeiro-Da-Silva">Alfredo Ribeiro-Da-Silva</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:52B25A2080D16025CDF04C23B7506C0125C357DD</idno>
<date when="2001" year="2001">2001</date>
<idno type="doi">10.1002/cne.1114</idno>
<idno type="url">https://api-v5.istex.fr/document/52B25A2080D16025CDF04C23B7506C0125C357DD/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002473</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002473</idno>
<idno type="wicri:Area/Istex/Curation">002473</idno>
<idno type="wicri:Area/Istex/Checkpoint">001038</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001038</idno>
<idno type="wicri:doubleKey">0021-9967:2001:Ruocco I:light:and:electron</idno>
<idno type="wicri:Area/Main/Merge">003691</idno>
<idno type="wicri:Area/Main/Curation">003209</idno>
<idno type="wicri:Area/Main/Exploration">003209</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Light and electron microscopic study of the distribution of substance P‐immunoreactive fibers and neurokinin‐1 receptors in the skin of the rat lower lip</title>
<author><name sortKey="Ruocco, Isabella" sort="Ruocco, Isabella" uniqKey="Ruocco I" first="Isabella" last="Ruocco">Isabella Ruocco</name>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pharmacology and Therapeutics, McGill University, Montreal H3G 1Y6, Quebec</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cuello, A Claudio" sort="Cuello, A Claudio" uniqKey="Cuello A" first="A. Claudio" last="Cuello">A. Claudio Cuello</name>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pharmacology and Therapeutics, McGill University, Montreal H3G 1Y6, Quebec</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Anatomy and Cell Biology, McGill University, Montreal H3G 1Y6, Quebec</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shigemoto, Ryuichi" sort="Shigemoto, Ryuichi" uniqKey="Shigemoto R" first="Ryuichi" last="Shigemoto">Ryuichi Shigemoto</name>
<affiliation wicri:level="1"><country xml:lang="fr">Japon</country>
<wicri:regionArea>National Institute of Physiological Sciences, Crest Japan Science and Technology Corporation, Okazaki 444‐8585</wicri:regionArea>
<wicri:noRegion>Okazaki 444‐8585</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ribeiro A Ilva, Alfredo" sort="Ribeiro A Ilva, Alfredo" uniqKey="Ribeiro A Ilva A" first="Alfredo" last="Ribeiro-Da-Silva">Alfredo Ribeiro-Da-Silva</name>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pharmacology and Therapeutics, McGill University, Montreal H3G 1Y6, Quebec</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Anatomy and Cell Biology, McGill University, Montreal H3G 1Y6, Quebec</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir‐William‐Osler, Montreal H3G 1Y6, Quebec</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Comparative Neurology</title>
<title level="j" type="abbrev">J. Comp. Neurol.</title>
<idno type="ISSN">0021-9967</idno>
<idno type="eISSN">1096-9861</idno>
<imprint><publisher>John Wiley & Sons, Inc.</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="2001-04-16">2001-04-16</date>
<biblScope unit="volume">432</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="466">466</biblScope>
<biblScope unit="page" to="480">480</biblScope>
</imprint>
<idno type="ISSN">0021-9967</idno>
</series>
<idno type="istex">52B25A2080D16025CDF04C23B7506C0125C357DD</idno>
<idno type="DOI">10.1002/cne.1114</idno>
<idno type="ArticleID">CNE1114</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0021-9967</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>blood vessels</term>
<term>dermis</term>
<term>epidermis</term>
<term>immunocytochemistry</term>
<term>mast cells</term>
<term>sensory fibers</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cutaneous antidromic vasodilatation and plasma extravasation, two phenomena that occur in neurogenic inflammation, are partially blocked by substance P (SP) receptor antagonists and are known to be mediated in part by mast cell‐released substances, such as histamine, serotonin, and nitric oxide. In an attempt to provide a morphological substrate for the above phenomena, we applied light and electron microscopic immunocytochemistry to investigate the pattern of SP innervation of blood vessels and its relationship to mast cells in the skin of the rat lower lip. Furthermore, we examined the distribution of SP (neurokinin‐1) receptors and their relationship to SP‐immunoreactive (IR) fibers. Our results confirmed that SP‐IR fibers are found in cutaneous nerves and that terminal branches are observed around blood vessels and penetrating the epidermis. SP‐IR fibers also innervated hair follicles and sebaceous glands. At the ultrastructural level, SP‐IR varicosities were observed adjacent to arterioles, capillaries, venules, and mast cells. The varicosities possessed both dense core vesicles and agranular synaptic vesicles. We quantified the distance between SP‐IR varicosities and blood vessel endothelial cells. SP‐IR terminals were located within 0.23–5.99 μm from the endothelial cell layer in 82.7% of arterioles, in 90.2% of capillaries, and in 86.9% of venules. Although there was a trend for SP‐IR fibers to be located closer to the endothelium of venules, this difference was not significant. Neurokinin‐1 receptor (NK‐1r) immunoreactivity was most abundant in the upper dermis and was associated with the wall of blood vessels. NK‐1r were located in equal amounts on the walls of arterioles, capillaries, and venules that were innervated by SP‐IR fibers. The present results favor the concept of a participation of SP in cutaneous neurogenic vasodilatation and plasma extravasation both by an action on blood vessels after binding to the NK‐1r and by causing the release of substances from mast cells after diffusion through the connective tissue. J. Comp. Neurol. 432:466–480, 2001. © 2001 Wiley‐Liss, Inc.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>Japon</li>
</country>
<region><li>Québec</li>
</region>
<settlement><li>Montréal</li>
</settlement>
<orgName><li>Université McGill</li>
</orgName>
</list>
<tree><country name="Canada"><region name="Québec"><name sortKey="Ruocco, Isabella" sort="Ruocco, Isabella" uniqKey="Ruocco I" first="Isabella" last="Ruocco">Isabella Ruocco</name>
</region>
<name sortKey="Cuello, A Claudio" sort="Cuello, A Claudio" uniqKey="Cuello A" first="A. Claudio" last="Cuello">A. Claudio Cuello</name>
<name sortKey="Cuello, A Claudio" sort="Cuello, A Claudio" uniqKey="Cuello A" first="A. Claudio" last="Cuello">A. Claudio Cuello</name>
<name sortKey="Ribeiro A Ilva, Alfredo" sort="Ribeiro A Ilva, Alfredo" uniqKey="Ribeiro A Ilva A" first="Alfredo" last="Ribeiro-Da-Silva">Alfredo Ribeiro-Da-Silva</name>
<name sortKey="Ribeiro A Ilva, Alfredo" sort="Ribeiro A Ilva, Alfredo" uniqKey="Ribeiro A Ilva A" first="Alfredo" last="Ribeiro-Da-Silva">Alfredo Ribeiro-Da-Silva</name>
<name sortKey="Ribeiro A Ilva, Alfredo" sort="Ribeiro A Ilva, Alfredo" uniqKey="Ribeiro A Ilva A" first="Alfredo" last="Ribeiro-Da-Silva">Alfredo Ribeiro-Da-Silva</name>
</country>
<country name="Japon"><noRegion><name sortKey="Shigemoto, Ryuichi" sort="Shigemoto, Ryuichi" uniqKey="Shigemoto R" first="Ryuichi" last="Shigemoto">Ryuichi Shigemoto</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003209 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003209 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Canada |area= ParkinsonCanadaV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:52B25A2080D16025CDF04C23B7506C0125C357DD |texte= Light and electron microscopic study of the distribution of substance P‐immunoreactive fibers and neurokinin‐1 receptors in the skin of the rat lower lip }}
This area was generated with Dilib version V0.6.29. |